mouse anti transforming growth factor β  (Santa Cruz Biotechnology)

Journal: PLoS ONE

Article Title: Aberrant Hedgehog Ligands Induce Progressive Pancreatic Fibrosis by Paracrine Activation of Myofibroblasts and Ductular Cells in Transgenic Zebrafish

doi: 10.1371/journal.pone.0027941

Figure Lengend Snippet: ( A ) IHC and ISH (inlets) showing MMP9 expression in proliferating myofibroblasts and ductular cells. ( B ) IHC for TGFß1. Contrary to MMP9, TGFß1 is expressed only in proliferating ductular cells which are also positive for cytokeratin. ( C, D ) ISH for PDGFAa and IL1b. Transcripts of both genes are detected in a small subset of proliferating myofibroblasts and ductular cells. ( A–C , red arrowheads, ductular cells). Microscopic images are 400×. Bars, 50 µm.

Article Snippet: Primary antibodies used for immunohistochemistry were rabbit anti-α-smooth muscle actin (α-SMA) (Abcam ab15734, 1∶500), rabbit anti-Smoothened (Smo) (Abcam ab72130, 1∶200), rabbit anti-Gli1 (Upstate AB3444, 1∶500), rabbit anti-Gli2 (Abcam ab26056, 1∶300), mouse anti-Transforming growth factor ß1 (TGFß1) (R&D MAB1835, 1∶500), rabbit anti-matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1∶500), mouse anti-cytokeratin (CK) AE1/AE3 (Abcam ab961, 1∶500), mouse anti-proliferating cell nuclear antigen (PCNA) (Abcam ab29, 1∶1000), and rabbit anti-phosphohistone H3 (pHH3) (Cell Signaling 9701, 1∶200).

Techniques: Expressing

Journal: Scientific Reports

Article Title: The potential role of the pseudobranch of molly fish ( Poecilia sphenops ) in immunity and cell regeneration

doi: 10.1038/s41598-023-34044-8

Figure Lengend Snippet: Immunohistochemical expressions of myogenin and TGF-β in the pseudobranch. ( A , B ) The PSCs (arrowheads) showed expressions for myogenin. ( C , D ) The PSCs (arrowheads) exhibited intense expressions for and TGF-β.

Article Snippet: The sections were incubated overnight at 4 °C with diluted primary antibodies against monoclonal anti-mouse transforming growth factor beta (TGf-β) (1:100, MA5-16949, Thermo Fischer Scientific, UK), nuclear factor kappa B (NF-κB) (10745-1-AP, Proteintech, USA), glial fibrillary acidic protein (GFAP) (PA5-16291, Thermo Fisher Scientific, Waltham, USA), autophagy protein 5 (APG5) (sc-133158, Santa Cruz Biotechnology, Heidelberg, Germany), a rabbit polyclonal anti-CD3 (cluster of differentiation 3) (1:200; Abcam, Cambridge, UK, ab828), mouse polyclonal anti-CD8 (1:200; Abcam, Cambridge, UK), a rabbit polyclonal antibody inducible nitric oxide synthase (iNOS-2) (RB-1605, Thermo Fisher Scientific, UK), mouse monoclonal CD45 (1:100, 14-0454-82, Thermo Fischer Scientific, UK), myogenin (AB3239, Sigma-Aldrich, Madrid, Spain), and SRY-Box transcription factor 9 (Sox9) (AB5535, Sigma-Aldrich, Madrid, Spain),SRY-Box transcription factor 9 (Sox9).

Techniques: Immunohistochemical staining

Journal: Scientific Reports

Article Title: The potential role of the pseudobranch of molly fish ( Poecilia sphenops ) in immunity and cell regeneration

doi: 10.1038/s41598-023-34044-8

Figure Lengend Snippet: The distribution of telocytes (TCs) in the pseudobranch. ( A ) Many TCs (arrowheads, boxed areas) are demonstrated in the connective tissue surrounding the lamellae. Note the presence of many melanocytes (arrows) in the connective tissue around the pseudobranch. (HE). ( B ) The cell processes of TCs (arrowheads) established a network and stained positive with PAS. ( C , D ) TCs (arrowheads) expressed SOX9 and TGF-β respectively.

Article Snippet: The sections were incubated overnight at 4 °C with diluted primary antibodies against monoclonal anti-mouse transforming growth factor beta (TGf-β) (1:100, MA5-16949, Thermo Fischer Scientific, UK), nuclear factor kappa B (NF-κB) (10745-1-AP, Proteintech, USA), glial fibrillary acidic protein (GFAP) (PA5-16291, Thermo Fisher Scientific, Waltham, USA), autophagy protein 5 (APG5) (sc-133158, Santa Cruz Biotechnology, Heidelberg, Germany), a rabbit polyclonal anti-CD3 (cluster of differentiation 3) (1:200; Abcam, Cambridge, UK, ab828), mouse polyclonal anti-CD8 (1:200; Abcam, Cambridge, UK), a rabbit polyclonal antibody inducible nitric oxide synthase (iNOS-2) (RB-1605, Thermo Fisher Scientific, UK), mouse monoclonal CD45 (1:100, 14-0454-82, Thermo Fischer Scientific, UK), myogenin (AB3239, Sigma-Aldrich, Madrid, Spain), and SRY-Box transcription factor 9 (Sox9) (AB5535, Sigma-Aldrich, Madrid, Spain),SRY-Box transcription factor 9 (Sox9).

Techniques: Staining

Journal: Scientific Reports

Article Title: The potential role of the pseudobranch of molly fish ( Poecilia sphenops ) in immunity and cell regeneration

doi: 10.1038/s41598-023-34044-8

Figure Lengend Snippet: Suppliers and dilutions of the first antibodies used.

Article Snippet: The sections were incubated overnight at 4 °C with diluted primary antibodies against monoclonal anti-mouse transforming growth factor beta (TGf-β) (1:100, MA5-16949, Thermo Fischer Scientific, UK), nuclear factor kappa B (NF-κB) (10745-1-AP, Proteintech, USA), glial fibrillary acidic protein (GFAP) (PA5-16291, Thermo Fisher Scientific, Waltham, USA), autophagy protein 5 (APG5) (sc-133158, Santa Cruz Biotechnology, Heidelberg, Germany), a rabbit polyclonal anti-CD3 (cluster of differentiation 3) (1:200; Abcam, Cambridge, UK, ab828), mouse polyclonal anti-CD8 (1:200; Abcam, Cambridge, UK), a rabbit polyclonal antibody inducible nitric oxide synthase (iNOS-2) (RB-1605, Thermo Fisher Scientific, UK), mouse monoclonal CD45 (1:100, 14-0454-82, Thermo Fischer Scientific, UK), myogenin (AB3239, Sigma-Aldrich, Madrid, Spain), and SRY-Box transcription factor 9 (Sox9) (AB5535, Sigma-Aldrich, Madrid, Spain),SRY-Box transcription factor 9 (Sox9).

Techniques:

Journal: Journal of Clinical Medicine

Article Title: Markers of Inflammation and Vascular Parameters in Selective Progesterone Receptor Modulator (Ulipristal Acetate)-Treated Uterine Fibroids

doi: 10.3390/jcm10163721

Figure Lengend Snippet: Representative light micrographs of immunoexpression (brown-stained cell cytoplasm indicated by black arrows) of inflammatory markers and vessel marker in fibroid cells in the control group and study groups after 3 months of treatment with ulipristal acetate (UPA): good, weak, and no response to treatment. Note: scale bar—100 µm. Abbreviations: CD31—marker of endothelial cells; CD68—marker of cells of the macrophage lineage; CD117—marker of mast cells; IL6—interleukin 6; IL10—interleukin 10; TGFβ 1—transforming growth factor β 1; and TNFα—tumor necrosis factor α.

Article Snippet: In order to determine the immunoexpression of the specific proteins, the following antibodies were used: (1) mouse monoclonal anti-transforming growth factor β antibody (Abcam, Cambridge, UK), diluted 1:200; (2) mouse polyclonal anti-tumor necrosis factor α antibody (Nordic BioSite, Täby, Sweden), diluted 1:300; (3) rabbit polyclonal anti-interleukin 6 antibody (Nordic BioSite, Täby, Sweden), diluted 1:200; (4) rabbit polyclonal anti-interleukin 10 antibody (Nordic BioSite, Täby, Sweden), diluted 1:200; (5) rabbit polyclonal anti-CD117 antibody (Dako, Glostrup, Denmark), diluted 1:500; (6) mouse monoclonal anti-CD68 antibody (Dako, Glostrup, Denmark), diluted 1:50; and (7) mouse monoclonal anti-CD31 antibody (Dako, Glostrup, Denmark), diluted 1:20.

Techniques: Staining, Marker